《英文msh词典》Mutageneses,Site-Specific
[入口词] Mutageneses,Site-Specific
[主题词] Mutagenesis,Site-Directed
[英文释义] Mutagenesis where the mutation is caused by in vitro induction directed at a specific site in a DNA molecule. The most common method involves use of a chemically synthesized oligonucleotide mutant which can hybridize with the DNA target molecule. The resulting mismatch-carrying DNA duplex may then be transfected into a bacterial cell line and the mutant strands recovered.