| 单词 | Chain Reaction,Ligase |
| 释义 | Chain Reaction,Ligase《英文msh词典》Chain Reaction,Ligase [入口词] Chain Reaction,Ligase [主题词] Ligase Chain Reaction [英文释义] A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA,(2) annealing of probes to target DNA,and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured. |
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